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PCR was used to amplify the different regions of the Jhamt or Cyp15A1 promoter. These variant fragments were then inserted into a linearized pGL3-basic vector (Promega, WI, USA). Pnt was cloned into a PIEX4 expression vector (Invitrogen, MA, USA) for overexpression in Drosophila KC cells. The pGL3 reporter vector carrying an indicated promoter region and a reference reporter plasmid of pRL-SV40 were co-transfected with PIEX4-Pnt-Flag or PIEX4-GFP (control) into KC cells and then incubated in 96-well plates at 28 °C for 48 h. Luciferase activity was normalized to Renilla luciferase activity and determined using the Dual-Luciferase® Reporter (DLR™) Assay System and a GloMax 96 Microplate Luminometer (Promega, WI, USA) [ 76]. EMSA Mittal H, Mishra SB, Mishra AK, Kaith BS, Jindal R (2013) Flocculation characteristics and biodegradation studies of Gum ghatti based hydrogels. Int J Biol Macromol 58:37–46 Hardy cited as his most important influence his independent study of Cours d'analyse de l'École Polytechnique by the French mathematician Camille Jordan, through which he became acquainted with the more precise mathematics tradition in continental Europe. In 1900 he passed part II of the Tripos, and in the same year he was elected to a Prize Fellowship at Trinity College. [1] :448 In 1903 he earned his M.A., which was the highest academic degree at English universities at that time. When his Prize Fellowship expired in 1906 he was appointed to the Trinity staff as a lecturer in mathematics, where teaching six hours per week left him time for research. [1] :448 In 1919 he left Cambridge to take the Savilian Chair of Geometry (and thus become a Fellow of New College [13]) at Oxford in the aftermath of the Bertrand Russell affair during World War I. Hardy spent the academic year 1928–1929 at Princeton University in an academic exchange with Oswald Veblen, who spent the year at Oxford. [3] Hardy gave the Josiah Willard Gibbs lecture for 1928. [14] [15] Hardy left Oxford and returned to Cambridge in 1931, becoming again a fellow of Trinity College and holding the Sadleirian Professorship until 1942. [1] :453 Miui F, Zhao Q, Lui L (2013) Experimental study on the electrokinetics of kaolinite particles in aqueous suspension. Physicochem Probl Miner Process 49:659–672 Bai H, Palli SR. Identification of G protein-coupled receptors required for vitellogenin uptake into the oocytes of the red flour beetle, Tribolium castaneum. Sci Rep UK. 2016;6:27648.Kai ZP, Yin Y, Zhang ZR, Huang J, Tobe SS, Chen SS. A rapid quantitative assay for juvenile hormones and intermediates in the biosynthetic pathway using gas chromatography tandem mass spectrometry. J Chromatogr A. 2018;1538:67–74. Sharma BR, Dhuldhoya NC, Merchant UC (2006) Flocculants—an ecofriendly approach. J Polym Environ 14:195–202

Godfrey Harold Hardy FRS [1] (7 February 1877 – 1 December 1947) [2] was an English mathematician, known for his achievements in number theory and mathematical analysis. [3] [4] In biology, he is known for the Hardy–Weinberg principle, a basic principle of population genetics.

Verma AK, Dash RR, Bhunia PA (2019) Review on chemical coagulation/flocculation technologies for removal of colour from textile wastewaters. J Environ Manag 93(1):154–168 Rajagopal R, Saady NMC, Torrijos M, Thanikal JV, Hung YT (2013) Sustainable agro-food industrial wastewater treatment using high rate anaerobic process. Water 5:292–311

Xing Y, Sun X, Li B (2009) Poly (methacrylic acid)-modified chitosan for enhancement adsorption of water-soluble cationic dyes. Polym Eng Sci 49:272–280

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with the standards required by law and use such information only for authorized plan administration purposes. Sen G, Kumar R, Ghosh S, Pal S (2009) A novel polymeric flocculant based on polyacrylamide grafted carboxymethyl starch. Carbohydr Polym 77:822–831 Soury R, Jabli M, Saleh TA, Azharkechich Loiseau F, Saint-Aman E, Nasri H (2019) Degradation of calmagite by dichloride (5,10,15,20 tetraphenylporphyrinato) antimony hexachloridoantimonate: [Sb(TPP)Cl2] SbCl6. Inorg Chem Commun 104:54–60 Santos CG, Fernandez-Nicolas A, Belles X. Smads and insect hemimetabolan metamorphosis. Dev Biol. 2016;417(1):104–13.