The Source Wholesale Colour Changing Clam Light

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Tomer, R., Khairy, K., Amat, F. & Keller, P. J. Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy. Nat. Methods 9, 755–763 (2012). Dean, K. M. & Fiolka, R. Lossless Three-dimensional parallelization in digitally scanned light-sheet fluorescence microscopy. Sci. Rep. 7, 9332 (2017). Ji, N., Milkie, D. E. & Betzig, E. Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues. Nat. Methods 7, 141–147 (2010).

As I mentioned in an earlier section, clamshell lighting, when done properly results in a double catchlight. In addition to being more closed between 2 PM and 4 AM, the clams also displayed frequent intermittent rapid valve closure events (“valve-clapping”) during that time, as indicated by sudden peaks in the measured voltage data. These events persisted for seconds or minutes in length and were longer in afternoon and nighttime hours when less or no light was present (< 50 μmol photons/m 2s). In the month of August, closures during the nighttime hours were shorter on average than they were during the daytime, but only Clam 1 showed a statistically significant difference (Mann-Whitney Wilcoxon test: W = 128578, p <0.001), while clam 2 and clam 3 did not reach significance (Clam 2: W = 2031498, p = 0.3; Clam 3: W = 408318, p = 0.089). Between May 3 rd and August 10 th, there were a mean 34 closures per day, but with great variability around that mean (SD = 27) ( Fig 4A). Abrahamsson, S. et al. Fast multicolor 3D imaging using aberration-corrected multifocus microscopy. Nat. Methods 10, 60–63 (2013).There’s no hard and fast rule to just how high and how far from the subject the light needs to be. The height should be above the subject’s head. Don’t position it too high or the light won’t create catchlights. Temperatures were maintained at a consistent 25°C, with diurnal variations of less than 0.25°C, while pH values ranged from 8.1–8.25 pH units, also following a diurnal pattern controlled by light-mediated photosynthetic activity in the tank. Similarly, DO varied between 6.89 and 9.94 mg/L, also following a diurnal pattern with peaks during the daytime hours. Chlorophyll-a and phycoerythrin values showed high variability, with extreme peaks in July and August approaching the detection limit of the instruments and corresponding to visible increases in water turbidity. In terms of light modifiers, unless you have a particularly harsh shot in mind, I’d suggest working with softboxes or beauty dishes. These will allow you to soften the light and create a more flattering result. Clamshell Lighting Basics This research was funded by King Abdullah University of Science and Technology (KAUST) baseline funding to CD. (BAS/1/1071-01-01) and BO (BAS/1/1614-01-01). Conflict of Interest

Voleti, V. et al. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nat. Methods 16, 1054–1062 (2019). Elfwing T, Plantman P, Tedengren M, Wijnbladh E. Responses to temperature, heavy metal and sediment stress by the giant clam Tridacna squamosa. Marine and Freshwater Behaviour and Physiology. 2001;34: 239–248. Pro tip: If you’re uncertain about the final look you want, you can always start with butterfly lighting. Experiment with the shadows and angles, then simply add a fill light to achieve the clamshell look if you desire something softer. This way, you can play around with two different lighting styles with very little effort. It’s a convenient and creative approach to portrait lighting. Clamshell vs Rembrandt lighting

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Fatherree JW. Giant Clams in the Reef Aquarium: Biology, Identification, and Care. Liquid Medium Publications; 2019. Jou L-J, Lin S-C, Chen B-C, Chen W-Y, Liao C-M. Synthesis and measurement of valve activities by an improved online clam-based behavioral monitoring system. Computers and electronics in agriculture. 2013;90: 106–118. Generally, the second (fill) light should be weaker than the main (key) light. The fill light certainly shouldn’t be stronger or closer than the key light, because this would produce grotesque shadows that come from beneath the subject, like you’d find in a horror movie.